Immunoassay Solutions for Neuroinflammation

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We Have the Right Immunoassay for Your

Neuroinflammation Research

Whether you need qualitative or quantitative results, have limited or abundant sample, require single or multianalyte analysis, you will be able to find an optimal solution at Bio-Techne for detecting cytokines in your neural samples.

Proteome Profi ler™ Antibody Arrays

R&D Systems® Proteome Profi ler™ Antibody Arrays are highly effi cient, qualitative tools that allow

researchers to quickly and inexpensively survey which cytokines and growth factors are present in cell culture supernatants and tissue homogenates.

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• No Specialized Equipment Needed

They are designed to utilize the same data collection equipment used for Western blots.

• Wide Selection

Choose from 29 arrays for the detection of both intracellular and extracellular factors from a broad variety of sample types.

• User-Friendly Design

Each array is stamped with an identifi cation number for easy record keeping and contains reference spots in three corners for orientation purposes.

• Multiple Detection Methods Available

Arrays can utilize either chemiluminescence or LI-COR® infrared fl uorescence detection.*

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Cytokine Expression Induced by IL-1b/IL-1F2 in Mouse Brain. C57BL/6 mice received a bilateral intrahippo campal injection of adeno-associated virus (AAV2) vector expressing either a single chain antibody to phenobarbital (for a control) or IL-1b/ IL-1F2. After 4 weeks, brains of the mice were collected, and cytokine expression in brain homogenates was analyzed using the Proteome Profi ler™ Mouse Cytokine Antibody Array (R&D Systems, Catalog # ARY006). Representative arrays (A) and

histogram profi les (B) for select analytes from control (gray bars) and IL-1b/IL-1F2 treated (orange bars) mice. Data were generated by analysis of the mean pixel density of individual antibody spots using image software analysis. Data courtesy of Dr. Jonathan Cherry, University of Rochester Medical Center, Rochester, NY.

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Cytokine Profi le of Cultured Human Astrocytes. Human primary astrocyte cell cultures were treated with recombinant human IL-1b/IL-1F2 and TNF-α for 24 hours or remained untreated. Conditioned media from the cultures were harvested and assayed for the presence of 36 cytokines using the Proteome Profi ler™ Human Cytokine

Antibody Array (R&D Systems, Catalog # ARY005). Cytokines that were newly expressed (red boxes) or whose expression changed (blue boxes) following treatment are highlighted. Image from Choi, S.S. et al. (2014) PLoS One 9:e92325.

Luminex® Bead-Based Assays

R&D Systems offers two bead-based multiplex immunoassay formats for detecting protein analytes in biological fl uids. These assays utilize Luminex® xMAP® microparticle technology

allowing users to better tailor assay selection to their individual research needs. Additionally, our Luminex® assays are specifi cally designed to optimize the benefi ts and overcome the

challenges of multiplexing.

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R&D Systems® Luminex® Assays

These assays are designed to maximize multiplexing capacity and fl exibility while maintaining assay specifi city.

• Most Flexible

Choose from over 340 analytes. All analytes are available in either the polystyrene or magnetic microparticle format.

• Largest Luminex® Multiplex Selection Available

These assays allow you to maximize the use of your samples by simultaneously analyzing up to 100 analytes.

• Unique Analytes Offered

Nearly 25% of our analytes are unique to us. R&D Systems® Luminex® High

Performance Assays

These assays are designed to maximize assay accuracy and precision while preserving the benefi ts of multiplexing.

• Most Accurate

Panel development and validation testing for these assays are similar to our gold-standard Quantikine® ELISA Assays.

• Flexible

Select panels are available

in either the polystyrene or magnetic microparticle format.

• Customizable to Fit Your Needs

Users can choose their analytes of interest from established panels and select “premixed” or “end-user mixed” options.

Simple Plex™ Assays

ProteinSimple® Simple Plex™ Assays are highly sensitive, reproducible immunoassays, in a single

or multianalyte cartridge format, that will transform your research possibilities. These novel assays offer the same specificity of single plex ELISAs, but with picogram/mL detection limits, a four-five log dynamic range, and CVs within 7%.

• Unmatched Performance

Quantify up to four analytes in a single 25 mL sample. Simple Plex™ assays are sensitive

enough to detect sup-picogram levels of protein, and have a four–five log dynamic range to help avoid sample dilution.

• Fast Results

Setup only takes five minutes. You simply pipette your samples into our cartridge and place it in the Ella instrument, which automates the entire assay for you. Data is ready in 60 minutes.

• Unrivaled Content

Over 110 human analytes are currently available. Powered by R&D Systems, the assay menu is unlimited.

DuoSet® ELISA Development Systems

R&D Systems® DuoSet® ELISA Development Systems allow you to develop the immunoassay that

you need using our gold-standard ELISA reagents. They are an economical alternative to buying separate antibodies and protein standards when complete kits are not an option.

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• Customizable to Fit Your Needs

Our ELISA development systems are adaptable to multiple platforms, such as Gyrolab™, MSD®,

Luminex®, and 384-well plates, and we offer tailored protocols and ancillary kits.

• Trustworthy Performance

Capture and detection antibodies are carefully selected to ensure their fl awless performance as a sandwich ELISA antibody pair.

• We Have What You Need

We offer the largest selection of analytes (>800) for the most species.

• Economical

Many analytes are offered in a smaller 5-plate pack.

Quantikine® ELISA Kits

R&D Systems® Quantikine® ELISA Kits are complete, fully validated, ready-to-run sandwich

ELISAs that are designed to measure the concentrations of natural or recombinant analytes. These kits can be used to measure proteins in cerebral spinal fl uid, as well as other neural tissue including brain homogenates. In-house manufacturing and extensive validation testing ensure these kits provide the highest levels of specifi city, accuracy, precision, and sensitivity, making them the industry gold standard.

• Confi dence with Controls

Our assays have controls with assigned ranges that confi rm assay performance.

• Unrivaled Reputation

Our ELISA kits are the most referenced on the market.

• High Specifi city

Every complete ELISA kit is extensively tested against related molecules and common interfering substances to ensure no cross-reactivity or interference.

• Accurate Detection of Natural Proteins

Our antibody pairs recognize the supplied recombinant standard and natural proteins in biological samples in a parallel manner.

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