웅비Brochure
[The Well BioSceince] VitroGel STEM (공식대리점: 웅비메디텍)[출처]
앤유뱃살
2021. 6. 9. 09:28
http://www.woongbee.com/0NewHome/Brochure/pdf/vitrogelstem.pdf
● Stem Cell Scale Up Culture (# VHM02)
- High Cell Viability and Excellent Cell Grow Rate
- High-quality 3D stem cells with full pluripotent properties
- Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
● 2D → 3D Culture 로 전환: More Good Growth rate, More High Cell viability / Scale Up
- Scale Up Graph 참조 (Cell Numbers / Day #)
● LN2 → Thawing 후 3D Culture : High Stem Cell Viability
- Day 경과 별 [LN2 직접추출 Stem Cell]의 Vision Image 참조
● VitroGel STEM VitroGel Organoid : 오가노이드 분화 전 단계 사용
Choice and Purpose (Organoid/Stem) |
Use VitroGel ORGANOID for organoid formation and differentiation | Use VitroGel STEM for organoid formation | ||
Differentiation Phase | stem cell spheroids | Endoderm differentiation | Mid-/Hindgut Differentiation | Organoid formation and maturation |
End Period | Day 0 | Day 3 | Day 5-7 | Long-term culture |
** This scheme is an example for human derived intestinal organoid
● 3D → 2D Culture 로 쉽게 전환
Procedure | FAB detail |
1) Send 3D spheroid to Matrigel coating plate → 2) A single spheroid 24 hrs after attached back to Matrigel |
Xeno-free |
Room temp. operation/ Ready-to-use | |
Lab automation friendly for HTS | |
Easy cell harvesting | |
Time and cost saving advantages |