[The Well BioSceince] VitroGel STEM (공식대리점: 웅비메디텍)[출처]

http://www.woongbee.com/0NewHome/Brochure/pdf/vitrogelstem.pdf

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● Stem Cell Scale Up Culture (# VHM02)

- High Cell Viability and Excellent Cell Grow Rate

- High-quality 3D stem cells with full pluripotent properties

- Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)

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● 2D → 3D Culture 로 전환: More Good Growth rate, More High Cell viability / Scale Up

- Scale Up Graph 참조 (Cell Numbers / Day #)

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● LN2 → Thawing 후 3D Culture : High Stem Cell Viability

- Day 경과 별 [LN2 직접추출 Stem Cell]의 Vision Image 참조

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● VitroGel STEM VitroGel Organoid : 오가노이드 분화 전 단계 사용

Choice and Purpose (Organoid/Stem)	Use VitroGel ORGANOID for organoid formation and differentiation			Use VitroGel STEM for organoid formation
Differentiation Phase	stem cell spheroids	Endoderm differentiation	Mid-/Hindgut Differentiation	Organoid formation and maturation
End Period	Day 0	Day 3	Day 5-7	Long-term culture

** This scheme is an example for human derived intestinal organoid

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● 3D → 2D Culture 로 쉽게 전환

Procedure	FAB detail
1) Send 3D spheroid to Matrigel coating plate ​ → ​ 2) A single spheroid 24 hrs after attached back to Matrigel	Xeno-free
	Room temp. operation/ Ready-to-use
	Lab automation friendly for HTS
	Easy cell harvesting
	Time and cost saving advantages

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