Tools for Autophagy Research

http://www.woongbee.com/0NewHome/Brochure/pdf/ToolsforAutophagyResearch.pdf

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✔ Tools for Autophagy Research

 

• Ab Markers            • Activators            • Inhibitors            • Detection Reagents

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✔ Autophagy

 

- 세포의 생존 및 항상성 유지를 위한 필수기작

- 다양한 연구분야 접목

- 퇴행성 뇌질환 분야의 key

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✔ Autophagy Development Process

 

Normal     →     Induction     →     Autophagosome Formation     →     Autophagosome Lysosome Fusion

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Chart One.

 

Autophagy Activators		Autophagy Inhibitors
Cat#	Products	Cat#	Products
1234/10	A23187, free acid	1334/10U	Bafilomycin Al
4095/50	Amiodarone	1544/10	(±)-Bay K8644
1231/5	Brefeldin A	4109/50	Chloroquine
4098/50	Carbamazepine	2656/10U	Concanamycin A
1126/100	Dexamethasone	4417/10	DBeQ
3093/10	Dorsomorphin	4545/1	E64d
3993/1	EB1089	1130/5	LY294002
0741/1	GF109203X	3977/50	3-Methyladenine
0681/10	L-690,330	1228/10	Nocodazole
1391/10	NF449	1190/10	Pepstatin A
4079/50	Niclosamide	1097/10	Taxol
0600/100	Nimodipine	1256/10	Vinblastine
0601/50	Nitrendipine	1232/1	Wortmannin
2930/1	PI103
1267/10	Pifithrin-alpha hydrobromide
1292/1	Rapamycin
1610/10	Rottierin
4297/10	SMER28
2706/10	Temozolomide
1138/1	Thapsigargin
4247/10	Torinl
3516/10	Tunicamycin
2815/100	Valproic acid, sodium salt
0654/1G	Verapamil

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Chart Two.

 

Process	Active, physiological or pathophysiological
Induction stimuli	Starvation, hypoxia, chemotherapy, growth factor deprivation
Morphological changes	Vacuolization, mass degradation of organelles & proteins
Molecular/biochemical changes	LC3I lipidation to LC3II, p62/SQ STM1 degradation, lysosomal activity
Clearance	Cekks gets cannibalized & the con tents recycle for survival of the tissue

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Chart Three.

 

Autophagosome
유발요인	[Stage/방해] Marker
Starvation Hypoxia ROS Infection Chemotherapy Others	Autolysosome 형성방해	Cargo recognition 방해	Autophagic cargo (Stage)	Lysosome fusion 방해	Autolysosome 형성방해	Secretion (Stage)
	PICALM (AD) a-syn,VPS35(PD) Htt (Huntington’s)	PICALM (AD) Htt (Huntington’s) SQSTMl/p62,OPTN (ALS)	​ Aβ,Tau (AD)	Lysosome fusion 방해 PS-l(AD) GBA, a-syn,VPS35(PD)	Autolysosome 형성방해 Tau, PICALM (AD) CHMP28B(ALS)	| A3(ad)

[Autophagosome 단계 진행방향]

 

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Chart Four.

 

LC3 Antibodies
Target	Catalog #	Species	Applications
LC3	NB100-2331	H, M, R +	WB, SW, FC,ICC,IHC,IP
	NBP1-19167	H, M, R +	WB, SW, FC,ICC,IHC
	NBP1-78964	H, M, R +	WB, ICC, IHC
	NBP2-24392	H, M, R +	WB, IHC
LC3B	NB100-2220	H, M, R +	WB, SW, ICCJHC, IP
	NB600-1384	H, M, R +	WB, SW, EM, FC, ICC, IHC
LC3C	NBP2-36664	H	WB, ICC, IHC
	NB110-74806	H	WB, IHC
LC3 Antibody Pack	NB910-40435	H, M	WB, IHC

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Chart Five.

 

Reagent Information
​	Machines available			​ Application
​	Fluorescent microscope	Flow cytometer	Microplate reader
DAPGreen	O	O	O	phagophore ~Autophagosome formation
DALGreen	O	X	X	Auto lysosome

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Chart Six.

 

Autophagosome 형성 방해 관련 Marker
Target	Catalog#	Species	Application
PICALM	NBP1-86658	H, M, R	WB,IHC, IHC-P
alpha-Synuclein	NBP2-15365	H, M, R +	WBJCC/IF, IHC,IHC-Fr, IHC-P
VPS35	NB100-1397	H, M, R +	WB, ICC/IF, PEP-ELISA
HTT	NBP1-44266	H, M, R	WB, ELISA, ICC/IFJHC
P62/SQSTM1	NBP1-42821	H, M, R	WB, ICC
	MAB8028	H, M, R	WB, SW, ICC
GBA	NBP2-66871	H, M, R	WB, IHC, IHC-P
	MAB7410	H	WB, Simple Western, ICC/IF
AP	NBP2-13075	H, M, R, Mk	WB, ELISA, ICC/IF, IHC (Fr, P, FrFl) IP
Tau [p Thrl81]	NB100-82245	H, M, R	WB, Simple Western, IHC, IHC-P
Tau	NBP2-25163	H, M, R	WB, ICC/IF, IHC
OPTN	NBP1-84682	H, M	WB, ICC/IF, IHC, IHC-Fr, IHC-P
PS-1	NB100-74510	H, M, R, P	WB, ELISA, ICC/IF, IHC, IHC-P, IP
	NB300-749	H, M, R, P	WB, ELISA, ICC/IF, IHC, IHC-P, IP
	AF7509	H, M, R	IHC, WB
CHMP2B	MAB7509	H	IHC,WB
	NBP2-15887	H, M	WB, ICC/IF, IHC, IHC-P